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Novel test helps faster detection of pathogens in lungs


A new molecular based method can detect pathogens mico-bacteria that cause tuberculosis , much faster than culture techniques-bases that used to take several weeks, according to a study.

Mycobacterium tuberculosis is the causative agent of tuberculosis – a potentially serious bacterial infectious disease that mainly affects the lungs – and was responsible for the deaths of about 1.5 million people around the world 2014.

team studied more than 6,800 samples from patients to examine molecular-based methods for the detection of mycobacterial pathogens.

The researchers developed a diagnostic algorithm to detect mycobacteria directly from the patient sample using genetic analysis.

Like many mycobacteria growing at a very slow pace, evidence-based bacterial cultures were long and took several weeks to complete.

later susceptibility testing to determine the appropriate medication is also used to take one to two weeks.

The results showed that the use of molecular detection methods, most patients know after one or two days if they have an infection with pathogens tuberculosis or nontuberculous mycobacteria – which can trigger lung infections , lymph node infections and skin diseases in patients with compromised immune systems.

did not find

New methods based on molecules to be as accurate as long techniques based on the crop.

In addition, molecular analysis makes it possible for the first time to also detect non-tuberculous mycobacteria directly from the patient sample within a few hours.

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This means that appropriate therapeutic measures can also be started much faster, the researchers said.

If the patient has an infection of tuberculosis, an additional molecular test is carried out with susceptibility testing of the main drugs against tuberculosis “rifampicin” and “INH”.

“This also showed that based molecular method predicts reliably the results of resistance based on culture. It is possible to obtain certainty much sooner if it is likely to succeed therapy with standard drugs chosen” said Peter Keller of the University of Zurich in Switzerland.

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